Journal: PLoS ONE

Article Title: Renal IL-18 Production Is Macrophage Independent During Obstructive Injury

doi: 10.1371/journal.pone.0047417

Figure Lengend Snippet: Photographs (magnification 400X) depicting macrophage (brown stain; arrows) in vehicle treated and macrophage depleted mice exposed to sham operation or one week of UUO. T = tubule; G = glomerulus.

Article Snippet: The tissues were then blocked with 1% bovine serum albumin (BSA) and incubated with a a rat anti-mouse macrophage antibody (F4/80; 1∶100, Angio-Proteomie, Boston, MA) for 90 min at room temperature.

Techniques: Staining

Journal: PLoS ONE

Article Title: Renal IL-18 Production Is Macrophage Independent During Obstructive Injury

doi: 10.1371/journal.pone.0047417

Figure Lengend Snippet: Renal cortical active IL-18 protein levels in vehicle treated and macrophage depleted mice exposed to sham operation or one week of UUO.

Article Snippet: The tissues were then blocked with 1% bovine serum albumin (BSA) and incubated with a a rat anti-mouse macrophage antibody (F4/80; 1∶100, Angio-Proteomie, Boston, MA) for 90 min at room temperature.

Techniques:

Journal: PLoS ONE

Article Title: Renal IL-18 Production Is Macrophage Independent During Obstructive Injury

doi: 10.1371/journal.pone.0047417

Figure Lengend Snippet: Quantitative IL-18 receptor (IL-18R) mRNA expression represented as a percentage of β-actin in vehicle treated and macrophage depleted mice exposed to sham operation or one week of UUO.

Article Snippet: The tissues were then blocked with 1% bovine serum albumin (BSA) and incubated with a a rat anti-mouse macrophage antibody (F4/80; 1∶100, Angio-Proteomie, Boston, MA) for 90 min at room temperature.

Techniques: Expressing

Journal: PLoS ONE

Article Title: Renal IL-18 Production Is Macrophage Independent During Obstructive Injury

doi: 10.1371/journal.pone.0047417

Figure Lengend Snippet: Gel photograph and densitometric analysis of active caspase-1 expression represented as a percentage of GAPDH in vehicle treated and macrophage depleted mice exposed to sham operation or one week of UUO.

Article Snippet: The tissues were then blocked with 1% bovine serum albumin (BSA) and incubated with a a rat anti-mouse macrophage antibody (F4/80; 1∶100, Angio-Proteomie, Boston, MA) for 90 min at room temperature.

Techniques: Expressing

Journal: PLoS ONE

Article Title: Renal IL-18 Production Is Macrophage Independent During Obstructive Injury

doi: 10.1371/journal.pone.0047417

Figure Lengend Snippet: A. Photographs (magnification 400X) depicting renal cortical IL-18 production (red) and distal tubular staining (Peanut Agglutinin; green) in vehicle treated and macrophage depleted mice exposed to sham operation or one week of UUO. White arrows indicate IL-18 staining overlying tubules. T = Tubules.

Article Snippet: The tissues were then blocked with 1% bovine serum albumin (BSA) and incubated with a a rat anti-mouse macrophage antibody (F4/80; 1∶100, Angio-Proteomie, Boston, MA) for 90 min at room temperature.

Techniques: Staining

Journal: PLoS ONE

Article Title: Renal IL-18 Production Is Macrophage Independent During Obstructive Injury

doi: 10.1371/journal.pone.0047417

Figure Lengend Snippet: A. Photographs (magnification 400X) depicting renal cortical IL-18R production (red) and distal tubular staining (Peanut Agglutinin; green) in vehicle treated and macrophage depleted mice exposed to sham operation or one week of UUO. White arrows indicate IL-18R staining overlying tubules. T = Tubules.

Article Snippet: The tissues were then blocked with 1% bovine serum albumin (BSA) and incubated with a a rat anti-mouse macrophage antibody (F4/80; 1∶100, Angio-Proteomie, Boston, MA) for 90 min at room temperature.

Techniques: Staining

Journal: Reproductive Sciences

Article Title: Progesterone Receptor–Mediated Actions Regulate Remodeling of the Cervix in Preparation for Preterm Parturition

doi: 10.1177/1933719116650756

Figure Lengend Snippet: Representative photomicrographs of macrophages (F4/80-stained) and neutrophils (GR-1-stained) with methyl green or hematoxylin counterstain in sections of cervix from mice on day 16.5 postbreeding or postpartum (PP*, indicates for Veh group postbreeding day 19 and for onapristone [Ona] and RU486 groups day 17 postbreeding). Mice were treated with vehicle (Veh) or PR antagonist (Ona or RU486).

Article Snippet: 52 Adjacent sections were processed by immunohistochemistry to identify F4/80-stained macrophages (1:800 dilution; product no. T-2006; Bachem, Torrance, California) or GR-1 neutrophils (1:100; MCA771G; Abd Serotec, Raleigh, North Carolina).

Techniques: Staining

Journal: Reproductive Sciences

Article Title: Progesterone Receptor–Mediated Actions Regulate Remodeling of the Cervix in Preparation for Preterm Parturition

doi: 10.1177/1933719116650756

Figure Lengend Snippet: Effects of PR antagonist treatment on cell nuclei (CN), density of macrophage, and neutrophils on days postbreeding and postpartum (PP). Groups and other designations are the same as in previous figures. Density of immune cells was normalized to CN to account for structural variability as detailed in the Methods section. Data are the mean ± standard error of the mean [SEM]. P < .05 vs aD15 controls (Con), bD16.5 vehicle-injected (Veh), cD16.5 onapristone (Ona), dD16.5 RU486, and fD17 Ona (analysis of variance [ANOVA]).

Article Snippet: 52 Adjacent sections were processed by immunohistochemistry to identify F4/80-stained macrophages (1:800 dilution; product no. T-2006; Bachem, Torrance, California) or GR-1 neutrophils (1:100; MCA771G; Abd Serotec, Raleigh, North Carolina).

Techniques: Injection

Journal: Reproductive Sciences

Article Title: Progesterone Receptor–Mediated Actions Regulate Remodeling of the Cervix in Preparation for Preterm Parturition

doi: 10.1177/1933719116650756

Figure Lengend Snippet: Effects of progestogen on density of resident macrophages and neutrophils in the cervix from groups of mice described in Figure 4. P < .05 versus aD15 controls (Con), bD16.5 Sham-operated (Sham), cD16.5 E + P ovariectomized (Ovx), dD16.5 R5020 Ovx, eD16.5 medroxyprogesterone acetate (MPA Ovx), fD17 Sham, gD18 Sham, hD18 mice ovariectomized with estradiol and progesterone (E + P Ovx) and D18.5 E-P Ovx (data not shown), iD18 R5020 Ovx, jD18 MPA Ovx, and oD16.5 mice ovariectomized with vehicle (Ovx + Veh).

Article Snippet: 52 Adjacent sections were processed by immunohistochemistry to identify F4/80-stained macrophages (1:800 dilution; product no. T-2006; Bachem, Torrance, California) or GR-1 neutrophils (1:100; MCA771G; Abd Serotec, Raleigh, North Carolina).

Techniques:

Journal: Journal of mammary gland biology and neoplasia

Article Title: Reelin Deficiency Delays Mammary Tumor Growth and Metastatic Progression

doi: 10.1007/s10911-017-9373-z

Figure Lengend Snippet: (a) F4/80 labeling of primary tumors from rlOrl −/− and rlOrl +/+ mice. Bar = 50 µm. (b) High magnification view of the areas outlined in (a). Bar = 20 µm. (c) Quantification of the area occupied by F4/80-positive cells, rlOrl +/+ n=6, rlOrl −/−: n=6. (d) qPCR analysis of iNOS (M1), Arg1 (M2), Mrc1 (M2), and IL-10 gene expression in primary tumors from rlOrl −/−mice, gene expression levels are relative to those in tumors from rlOrl +/+ controls. (e) Western blot of Arg1 and β-actin expression in primary tumors from rlOrl −/− and rlOrl +/+mice. (f) Quantification of band intensities in (e). *P < 0.05, **P < 0.01, ***P < 0.001. Statistical significance determined using two-tailed, unpaired Student’s t-test.

Article Snippet: The antibodies used were rat anti-F4/80 (1:1000, Abbiotec), Alexa Fluor 594 donkey anti-rat (1:500, Life Technologies).

Techniques: Labeling, Expressing, Western Blot, Two Tailed Test